Tawfick A. et al., 2022: Predictive urinary RNA biomarkers of kidney injury after extracorporeal shock wave lithotripsy.
Tawfick A, Matboli M, Shamloul S, Agwa SHA, Saad M, Shaker H, Selim MMY, Salim MS, Radwan A, Shorbagy AA, Mousa W.
Urology Department, Faculty of Medicine, Ain Shams University, Cairo, 11381, Egypt.
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, 11381, Egypt.
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, 11381, Egypt.
Clinical Pathology and Molecular Genomics Unit, Medical Ain Shams Research Institute, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Medical Biochemistry and Molecular Biology Department, Modern University for Technology and Information, Cairo, Egypt.
Urology Department, Faculty of Medicine, Ain Shams University, Cairo, 11381, Egypt.
Abstract
Background: Extracorporeal shock wave lithotripsy (ESWL) is considered one of the best choices for the treatment of various kinds of urinary tract calculi, although it might cause acute kidney injury.
Objective: To measure the urinary long non-coding RNA-messenger RNA (LncRNA-mRNA) panel before and after ESWL to evaluate post-ESWL renal injury in a reliable and non-invasive method.
Patients and methods: The study included 60 patients with renal stones treated with ESWL and 30 healthy volunteers. Voided urine samples were obtained before, 2 h, and 1 day after ESWL. We measured the urinary level of LncRNA (SBF2-AS1, FENDRR-19) and mRNA (GBP1, NLRP3) by real-time qPCR and compared the results with serum creatinine and eGFR.
Results: LncRNA (SBF2-AS1, FENDRR-19) and mRNA (GBP1, NLRP3) levels were higher in patients with renal stones when compared with healthy volunteers. They showed a statistically significant increase in the level of LncRNA-mRNA panel in baseline and after ESWL treatment.
Conclusion: LncRNA (SBF2-AS1, FENDRR-19) and mRNA (GBP1, NLRP3) levels were significantly elevated following ESWL treatment, highlighting the usefulness of urinary biomarkers in identifying patients at higher risk of developing renal injury after ESWL treatment.
World J Urol. 2022 Jun;40(6):1561-1567. doi: 10.1007/s00345-022-03996-3. Epub 2022 Apr 15. PMID: 35428927. FREE ARTICLE
Comments 1
ESWL causes changes of renal tissue equivalent to a grade 1 contusion, sometimes less, seldom more. It is to be expected and has been demonstrated in many studies that certain markers indicating tissue trauma are elevated after ESWL. The present study is no exemption. However, I do not find the conclusion substantiated by the study. Neither did the authors show that a preoperative higher LncRNA-mRNA level lead to a higher “renal injury” nor were the patients evaluated more than 24 after ESWL to demonstrate “renal injuries” except the elevated LncRNA-mRNA levels at day 1.
Peter Alken